Isolation, Characterization, Kinetics, and Enzymatic and Nonenzymatic Microbicidal Activities of a Novel c-Type Lysozyme from Plasma of Schistocerca gregaria (Orthoptera: Acrididae) - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Journal of Insect Science Année : 2015

Isolation, Characterization, Kinetics, and Enzymatic and Nonenzymatic Microbicidal Activities of a Novel c-Type Lysozyme from Plasma of Schistocerca gregaria (Orthoptera: Acrididae)

Résumé

A protein, designated as Sgl, showing a muramidase lytic activity to the cell wall of the Gram-positive bacterium Micrococcus lysodeikticus was isolated for the first time from plasma of Escherichia coli-immunized fifth instar Schistocerca gregaria. The isolated Sgl was detected as a single protein band, on both native-and SDS-PAGE, has a molecular weight of similar to 15.7 kDa and an isoelectric point (pI) of ca 9.3 and its antiserum has specifically recognized its isolated form. Fifty-nine percentage of Sgl lytic activity was recovered in the isolated fractions and yielded ca 126-fold increase in specific activity than that of the crude. The partial N-terminal amino acid sequence of the Sgl has 55 and 40% maximum identity with Bombyx mori and Gallus gallus c-type lysozymes, respectively. The antibacterial activity against the Gram-positive and the Gram-negative bacteria were comparatively stronger than that of the hen egg white lysozyme (HEWL). The detected Sgl poration to the inner membrane that reach a maximum ability after 3 h was suggested to operate as a nonenzymatic mechanism for Gram-negative bacterial cell lysis, as tested in a permease-deficient E. coli, ML 35 strain. Sgl showed a maximal muramidase activity at pH 6.2, 30-50 degrees C, and 0.05M Ca2+ or Mg2+; and has a K-m of 0.5 mu g/ml and a V-max of 0.518 with M. lysodeikticus as a substrate. The Sgl displayed a chitinase activity against chitin with a K-m of 0.93 mg/ml and a V-max of 1.63.
Fichier principal
Vignette du fichier
Elmogy 2015 J. of Insect Science_{7B7F9B70-0517-4A57-9D29-7E84B7167812}.pdf (653.62 Ko) Télécharger le fichier
Origine : Accord explicite pour ce dépôt
Loading...

Dates et versions

hal-01165742 , version 1 (19-06-2015)
hal-01165742 , version 2 (21-07-2015)

Identifiants

Citer

Mohamed Elmogy, Taha T. M. Bassal, Hesham A. Yousef, Moataza A. Dorrah, Amr A. Mohamed, et al.. Isolation, Characterization, Kinetics, and Enzymatic and Nonenzymatic Microbicidal Activities of a Novel c-Type Lysozyme from Plasma of Schistocerca gregaria (Orthoptera: Acrididae). Journal of Insect Science, 2015, 15 (1), 10 p. ⟨10.1093/jisesa/iev038⟩. ⟨hal-01165742v2⟩
160 Consultations
116 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More