Vitamin A contained in the lipid droplets of rat liver stellate cells is substrate for acid retinyl ester hydrolase. - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue BBA - Biochimica et Biophysica Acta Année : 1995

Vitamin A contained in the lipid droplets of rat liver stellate cells is substrate for acid retinyl ester hydrolase.

Résumé

Vitamin A is stored in the lipid droplets of liver stellate cells (LSCs), as retinyl esters whose hydrolysis is necessary for the secretion of retinol into the blood. Here, we isolated these retinyl esters under their physiological form, i.e., in LSC lipid droplets, which had retained their morphological and biochemical characteristics. These retinyl esters are substrate for an hydrolytic enzyme, whose optimum pH is 4.1, and which is kinetically similar to the acidic retinyl ester hydrolase (aREH) we had previously described (Mercier et al., Biochim. Biophys. Acta (1994) 1212, 176-182). The cellular and subcellular localizations of aREH activity in rat liver suggest that this enzyme could be involved in the hydrolysis of the esterified vitamin A stores.

Dates et versions

hal-02713862 , version 1 (01-06-2020)

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Citer

Véronique Azaïs-Braesco, Isabelle Dodeman, Serge S. Delpal, Marie Cécile Alexandre-Gouabau, Anne Partier, et al.. Vitamin A contained in the lipid droplets of rat liver stellate cells is substrate for acid retinyl ester hydrolase.. BBA - Biochimica et Biophysica Acta, 1995, 1259 (3), pp.271-276. ⟨10.1016/0005-2760(95)00173-5⟩. ⟨hal-02713862⟩

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