Antibiotic marker modifications of lambda Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains. - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Journal of Microbiological Methods Année : 2008

Antibiotic marker modifications of lambda Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains.

Résumé

The Red recombinase system of bacteriophage Lambda has been used to inactivate chromosomal genes in bacteria using PCR products. In this study, we describe the replacement of the ampicillin resistance marker of helper plasmids pKD46 and pCP20 by a gentamicin resistance gene to disrupt chromosomal genes and then to eliminate FRT flanked resistance gene in multiple antibiotic-resistant Salmonella enterica strains.

Dates et versions

hal-00375588 , version 1 (15-04-2009)

Identifiants

Citer

Benoît Doublet, Gregory Douard, Hayette Targant, Danièle Meunier, Jean-Yves Madec, et al.. Antibiotic marker modifications of lambda Red and FLP helper plasmids, pKD46 and pCP20, for inactivation of chromosomal genes using PCR products in multidrug-resistant strains.. Journal of Microbiological Methods, 2008, 75 (2), pp.359-61. ⟨10.1016/j.mimet.2008.06.010⟩. ⟨hal-00375588⟩

Collections

ANSES INRA INRAE
332 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More