Malate-mediated carbon catabolite repression in bacillus subtilis involves the HPrK/CcpA pathway - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue The Journal of applied bacteriology Année : 2011

Malate-mediated carbon catabolite repression in bacillus subtilis involves the HPrK/CcpA pathway

Résumé

Most organisms can choose their preferred carbon source from a mixture of nutrients. This process is called carbon catabolite repression. The Gram-positive bacterium Bacillus subtilis uses glucose as the preferred source of carbon and energy. Glucose-mediated catabolite repression is caused by binding of the CcpA transcription factor to the promoter regions of catabolic operons. CcpA binds DNA upon interaction with its cofactors HPr(Ser-P) and Crh(Ser-P). The formation of the cofactors is catalyzed by the metabolite-activated HPr kinase/ phosphorylase. Recently, it has been shown that malate is a second preferred carbon source for B. subtilis that also causes catabolite repression. In this work, we addressed the mechanism by which malate causes catabolite repression. Genetic analyses revealed that malate-dependent catabolite repression requires CcpA and its cofactors. Moreover, we demonstrate that HPr(Ser-P) is present in malate-grown cells and that CcpA and HPr interact in vivo in the presence of glucose or malate but not in the absence of a repressing carbon source. The formation of the cofactor HPr(Ser-P) could be attributed to the concentrations of ATP and fructose 1,6-bisphosphate in cells growing with malate. Both metabolites are available at concentrations that are sufficient to stimulate HPr kinase activity. The adaptation of cells to environmental changes requires dynamic metabolic and regulatory adjustments. The repression strength of target promoters was similar to that observed in steady-state growth conditions, although it took somewhat longer to reach the second steady-state of expression when cells were shifted to malate.
Fichier principal
Vignette du fichier
6939.full_1.pdf (1.51 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)
Loading...

Dates et versions

hal-01019483 , version 1 (29-05-2020)

Identifiants

Citer

Frederik M. Meyer, Matthieu Jules, Felix M. P. Mehne, Dominique Le Coq, Jens J. Landmann, et al.. Malate-mediated carbon catabolite repression in bacillus subtilis involves the HPrK/CcpA pathway. The Journal of applied bacteriology, 2011, 193 (24), pp.6939 - 6949. ⟨10.1128/JB.06197-11⟩. ⟨hal-01019483⟩
46 Consultations
91 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More