RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design
Olle Terenius
(1)
,
Alexie Papanicolaou
(2, 3)
,
Jennie S. Garbutt
(4)
,
Ioannis Eleftherianos
(5)
,
Hanneke Huvenne
(6)
,
Sriramana Kanginakudru
(7)
,
Merete Albrechtsen
(8)
,
Chunju An
(9)
,
Jean-Luc Aymeric
(10)
,
Andrea Barthel
(11)
,
Piotr Bebas
(12)
,
Kavita Bitra
(11)
,
Alejandra Bravo
(13)
,
Francois Chevalieri
(14)
,
Derek P. Collinge
(3, 15)
,
Cristina M. Crava
(16)
,
Ruud A. de Maagd
(17)
,
Bernard Duvic
(10)
,
Martin Erlandson
(18, 19)
,
Ingrid Faye
(20)
,
Gabriella Felfoeldi
(21)
,
Haruhiko Fujiwara
(22)
,
Ryo Futahashi
(23, 24)
,
Archana S. Gandhe
(7)
,
Heather S. Gatehouse
(25)
,
Laurence N. Gatehouse
(25)
,
Jadwiga M. Giebultowicz
(26)
,
Isabel Gómez
(13)
,
Cornelis J. P. Grimmelikhuijzen
(27)
,
Astrid T. Groot
(11)
,
Frank Hauser
(27)
,
David G. Heckel
(11)
,
Dwayne D. Hegedus
(18)
,
Steven Hrycaj
,
Lihua Huang
,
J. Joe Hull
,
Kostas Iatrou
,
Masatoshi Iga
(6)
,
Michael R. Kanost
(9)
,
Joanna Kotwica
(12)
,
Changyou Li
,
Jianghong Li
,
Jisheng Liu
(6)
,
Magnus Lundmark
(8)
,
Shogo Matsumoto
,
Martina Meyering-Vos
,
Peter J. Millichap
(4)
,
Antonia Monteiro
,
Nirotpal Mrinal
(7)
,
Teruyuki Niimi
,
Daniela Nowara
(27)
,
Atsushi Ohnishi
,
Vicencio Oostra
,
Katsuhisa Ozaki
,
Maria Papakonstantinou
,
Aleksandar Popadic
,
Manchikatla V. Rajam
,
Suzanne Saenko
,
Robert M. Simpson
(25)
,
Mario Soberon
(13)
,
Michael R. Strand
(11)
,
Shuichiro Tomita
,
Umut Toprak
(19)
,
Ping Wang
,
Choon Wei Wee
,
Steven Whyard
,
Wenqing Zhang
,
Javaregowda Nagaraju
(7)
,
Richard H. Ffrench-Constant
(2)
,
Salvador Herrero
(16, 17)
,
Karl Gordon
(3)
,
Luc Swelters
,
Guy Smagghe
(6)
1
Dept Ecol
2 University of Exeter
3 CSIRO - Commonwealth Scientific and Industrial Research Organisation [Canberra]
4 University of Bath [Bath]
5 GW - The George Washington University
6 UGENT - Universiteit Gent = Ghent University
7 CDFD - Centre for DNA Fingerprinting and Diagnostics
8 Dept Plant Biol & Biotechnol
9 Kansas State University
10 Diversité, Génomes et Interactions Microorganismes-Insectes
11 Dept Entomol.
12 UW - University of Warsaw
13 UNAM - Universidad Nacional Autónoma de México = National Autonomous University of Mexico
14 UM2 - Université Montpellier 2 - Sciences et Techniques
15 Res Sch Biol
16 UV - Universitat de València
17 WUR - Wageningen University and Research Centre
18 AAFC - Agriculture and Agri-Food
19 U of S - University of Saskatchewan [Saskatoon]
20 Stockholm University
21 ELTE - Eötvös Loránd University
22 UTokyo - The University of Tokyo
23 Tokyo Metropolitan University
24 AIST - National Institute of Advanced Industrial Science and Technology
25 Plant & Food Research
26 OSU - Oregon State University
27 UCPH - University of Copenhagen = Københavns Universitet
2 University of Exeter
3 CSIRO - Commonwealth Scientific and Industrial Research Organisation [Canberra]
4 University of Bath [Bath]
5 GW - The George Washington University
6 UGENT - Universiteit Gent = Ghent University
7 CDFD - Centre for DNA Fingerprinting and Diagnostics
8 Dept Plant Biol & Biotechnol
9 Kansas State University
10 Diversité, Génomes et Interactions Microorganismes-Insectes
11 Dept Entomol.
12 UW - University of Warsaw
13 UNAM - Universidad Nacional Autónoma de México = National Autonomous University of Mexico
14 UM2 - Université Montpellier 2 - Sciences et Techniques
15 Res Sch Biol
16 UV - Universitat de València
17 WUR - Wageningen University and Research Centre
18 AAFC - Agriculture and Agri-Food
19 U of S - University of Saskatchewan [Saskatoon]
20 Stockholm University
21 ELTE - Eötvös Loránd University
22 UTokyo - The University of Tokyo
23 Tokyo Metropolitan University
24 AIST - National Institute of Advanced Industrial Science and Technology
25 Plant & Food Research
26 OSU - Oregon State University
27 UCPH - University of Copenhagen = Københavns Universitet
Bernard Duvic
- Fonction : Auteur
- PersonId : 735204
- IdHAL : bernard-duvic
- ORCID : 0000-0002-7618-7719
- IdRef : 033715645
Steven Hrycaj
- Fonction : Auteur
Lihua Huang
- Fonction : Auteur
J. Joe Hull
- Fonction : Auteur
- PersonId : 800746
- ORCID : 0000-0001-7991-3045
Kostas Iatrou
- Fonction : Auteur
Changyou Li
- Fonction : Auteur
Jianghong Li
- Fonction : Auteur
Shogo Matsumoto
- Fonction : Auteur
Martina Meyering-Vos
- Fonction : Auteur
Antonia Monteiro
- Fonction : Auteur
Teruyuki Niimi
- Fonction : Auteur
Atsushi Ohnishi
- Fonction : Auteur
Vicencio Oostra
- Fonction : Auteur
- PersonId : 784337
- ORCID : 0000-0002-1273-1906
Katsuhisa Ozaki
- Fonction : Auteur
Maria Papakonstantinou
- Fonction : Auteur
Aleksandar Popadic
- Fonction : Auteur
Manchikatla V. Rajam
- Fonction : Auteur
Suzanne Saenko
- Fonction : Auteur
Shuichiro Tomita
- Fonction : Auteur
Ping Wang
- Fonction : Auteur
Choon Wei Wee
- Fonction : Auteur
Steven Whyard
- Fonction : Auteur
Wenqing Zhang
- Fonction : Auteur
Salvador Herrero
- Fonction : Auteur
- PersonId : 784787
- ORCID : 0000-0001-5690-2108
Karl Gordon
- Fonction : Auteur
- PersonId : 761960
- ORCID : 0000-0001-5340-6774
- IdRef : 224512633
Luc Swelters
- Fonction : Auteur
Résumé
Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at http://insectacentral.org/RNAi will continue to gather information on RNAi experiments. (C) 2010 Elsevier Ltd. All rights reserved.