Type Three Effector Gene Distribution and Sequence Analysis Provide New Insights into the Pathogenicity of Plant-Pathogenic Xanthomonas arboricola - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Applied and Environmental Microbiology Année : 2012

Type Three Effector Gene Distribution and Sequence Analysis Provide New Insights into the Pathogenicity of Plant-Pathogenic Xanthomonas arboricola

Joël F. Pothier
  • Fonction : Auteur
Marion Fischer-Le Saux
Sophie Bonneau
  • Fonction : Auteur
  • PersonId : 1203345
Stéphane Poussier
  • Fonction : Auteur correspondant
  • PersonId : 970236

Connectez-vous pour contacter l'auteur
Tristan Boureau
  • Fonction : Auteur
  • PersonId : 1203052
Charles Manceau
  • Fonction : Auteur
  • PersonId : 942836

Résumé

Xanthomonas arboricola is a complex bacterial species which mainly attacks fruit trees and is responsible for emerging diseases in Europe. It comprises seven pathovars (X. arboricola pv. pruni, X. arboricola pv. corylina, X. arboricola pv. juglandis, X. arboricola pv. populi, X. arboricola pv. poinsettiicola, X. arboricola pv. celebensis, and X. arboricola pv. fragariae), each exhibiting characteristic disease symptoms and distinct host specificities. To better understand the factors underlying this ecological trait, we first assessed the phylogenetic relationships among a worldwide collection of X. arboricola strains by sequencing the housekeeping gene rpoD. This analysis revealed that strains of X. arboricola pathovar populi are divergent from the main X. arboricola cluster formed by all other strains. Then, we investigated the distribution of 53 type III effector (T3E) genes in a collection of 57 X. arboricola strains that are representative of the main X. arboricola cluster. Our results showed that T3E repertoires vary greatly between X. arboricola pathovars in terms of size. Indeed, X. arboricola pathovars pruni, corylina, and juglandis, which are responsible for economically important stone fruit and nut diseases in Europe, harbored the largest T3E repertoires, whereas pathovars poinsettiicola, celebensis, and fragariae harbored the smallest. We also identified several differences in T3E gene content between X. arboricola pathovars pruni, corylina, and juglandis which may account for their differing host specificities. Further, we examined the allelic diversity of eight T3E genes from X. arboricola pathovars. This analysis revealed very limited allelic variations at the different loci. Altogether, the data presented here provide new insights into the evolution of pathogenicity and host range of X. arboricola and are discussed in terms of emergence of new diseases within this bacterial species.
Fichier principal
Vignette du fichier
Type_three_effector_gene_distribution.pdf (1.51 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-01199341 , version 1 (18-06-2018)

Identifiants

Citer

Ahmed Hajri, Joël F. Pothier, Marion Fischer-Le Saux, Sophie Bonneau, Stéphane Poussier, et al.. Type Three Effector Gene Distribution and Sequence Analysis Provide New Insights into the Pathogenicity of Plant-Pathogenic Xanthomonas arboricola. Applied and Environmental Microbiology, 2012, 78 (2), pp.371--384. ⟨10.1128/AEM.06119-11⟩. ⟨hal-01199341⟩
159 Consultations
214 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More