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Article Dans Une Revue Applied and Environmental Microbiology Année : 2008

Strategy for in situ detection of natural transformation-based horizontal gene transfer events

Résumé

A strategy is described that enables the in situ detection of natural transformation in Acinetobacter baylyi BD413 by the expression of a green fluorescent protein. Microscale detection of bacterial transformants growing on plant tissues was shown by fluorescence microscopy and indicated that cultivation-based selection of transformants on antibiotic-containing agar plates underestimates transformation frequencies.

Dates et versions

hal-00312918 , version 1 (26-08-2008)

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Citer

Aurora Rizzi, Alessandra Pontiroli, Lorenzo Brusetti, Sara Borin, Claudia Sorlini, et al.. Strategy for in situ detection of natural transformation-based horizontal gene transfer events. Applied and Environmental Microbiology, 2008, 74 (4), pp.1250-1254. ⟨10.1128/AEM.02185-07⟩. ⟨hal-00312918⟩
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