We here report on the determination of the carbon isotopic composition of miliacin (olean-18-en-3βol methyl ether), extracted from the sediments of Lake le Bourget (French Alps). It is compared to the δ13C of miliacin extracted from Panicum miliaceum (broomcorn millet, a C4 plant) and Chionochloa sp. (a C3 plant). The δ13C of sedimentary miliacin (-21.5 ‰) is very close to that of miliacin extracted from bran (-23 ‰) and seeds (-23.5 ‰) of P. miliaceum and significantly different from that of Chionochloa sp. (-33 ‰). These results provide additional support for the use of sedimentary miliacin as a tracer of broomcorn millet, a C4 cereal cultivated since the Bronze Age around Lake le Bourget. These findings illustrate the potential of this compound to reconstruct past agriculture from lake sediment archives. Finally, considering the high abundances of miliacin in the bran of P. miliaceum this compound could have been wind-transported to the sediment during threshing and winnowing on the lake shore.