Production of olfactory receptor and nanosomes using yeast system for biolectronic nose
Résumé
Olfactory receptors (ORs) constitute the largest multigenic G protein-coupled receptor family, and are involved in the recognition of thousands of odorant molecules. However, if most of these ORs are identified on the basis of their DNA sequences, they are still unmatched to their natural ligands, and their deorphanization remains a challenging bottleneck. The identify odorant olfactory receptor pairs, various techniques have been developed to clone and produce ORs in cells, to allow the screening of their response to odorants. During the last decade, various ORs were successfully expressed in S. cerevisiae, and many improvements achieved. This chapter reviews the latest developments of yeast-based technologies to produce ORs and test their functional response.