Genome sequencing and analysis of the versatile cell factory Aspergillus niger CBS 513.88
Herman Pel
(1)
,
Johannes de Winde
(2, 1)
,
David Archer
(3)
,
Paul Dyer
(3)
,
Gerald Hofmann
(4)
,
Peter Schaap
(5)
,
Geoffrey Turner
(6)
,
Ronald de Vries
(7)
,
Richard Albang
(8)
,
Kaj Albermann
(8)
,
Mikael Andersen
(4)
,
Jannick Bendtsen
(9)
,
Jacques Benen
(5)
,
Marco van den Berg
(1)
,
Stefaan Breestraat
(1)
,
Mark Caddick
(10)
,
Roland Contreras
(11, 12)
,
Michael Cornell
(13)
,
Pedro Coutinho
(14)
,
Etienne Danchin
(14)
,
Alfons Debets
(15)
,
Peter Dekker
(1)
,
Piet van Dijck
(1)
,
Alard van Dijk
(1)
,
Lubbert Dijkhuizen
(16)
,
Arnold Driessen
(16)
,
Christophe D'Enfert
(17, 18)
,
Steven Geysens
(11)
,
Coenie Goosen
(19)
,
Gert Groot
(1)
,
Piet de Groot
(20)
,
Thomas Guillemette
(21)
,
Bernard Henrissat
(14)
,
Marga Herweijer
(1)
,
Johannes van den Hombergh
(1)
,
Cees van den Hondel
(22)
,
Rene van Der Heijden
(23)
,
Rachel van Der Kaaij
(19)
,
Frans Klis
(20)
,
Harrie Kools
(5)
,
Christian Kubicek
(24)
,
Patricia van Kuyk
(22)
,
Jürgen Lauber
(25)
,
Xin Lu
(24)
,
Marc van den Maarel
(19)
,
Rogier Meulenberg
(1)
,
Hildegard Menke
(1)
,
Martin Mortimer
(1)
,
Jens Nielsen
(26)
,
Stephen Oliver
(13)
,
Maurien Olsthoorn
(1)
,
Karoly Pal
(15, 27)
,
Noël van Peij
(1)
,
Arthur Ram
(22)
,
Ursula Rinas
(28)
,
Johannes Roubos
(1)
,
Cees Sagt
(1)
,
Monika Schmoll
(24)
,
Jibin Sun
(28)
,
David Ussery
(29)
,
János Varga
(27, 30)
,
Wouter Vervecken
(11, 12)
,
Peter van de Vondervoort
(22)
,
Holger Wedler
(25)
,
Han Wösten
(7)
,
An-Ping Zeng
(28)
,
Albert van Ooyen
(1)
,
Jaap Visser
(31)
,
Hein Stam
(1)
1
DSM
2 TU Delft - Delft University of Technology
3 School of Biology
4 DTU - Danmarks Tekniske Universitet = Technical University of Denmark
5 WUR - Wageningen University and Research Centre
6 Department of Molecular Biology and Biotechnology
7 Universiteit Utrecht / Utrecht University [Utrecht]
8 Biomax Informatics AG
9 CLC bio
10 School of Biological Sciences
11 UGENT - Universiteit Gent = Ghent University
12 Flanders Institute for Biotechnology
13 University of Manchester [Manchester]
14 AFMB - Architecture et fonction des Macromolécules Biologiques - UMR 6098
15 Laboratory of Genetics
16 University of Groningen [Groningen]
17 BPF - Biologie et Pathogénicité fongiques
18 IP - Institut Pasteur [Paris]
19 Centre for Carbohydrate Bioprocessing
20 VU - Vrije Universiteit Amsterdam [Amsterdam]
21 Unité de recherche Pathologie végétale et phytobactériologie
22 Universiteit Leiden = Leiden University
23 Radboud University Medical Center [Nijmegen]
24 Vienna University of Technology
25 Qiagen GmbH
26 CMB - Center for Microbial Biotechnology
27 University of Szeged [Szeged]
28 HZI - Helmholtz Centre for Infection Research
29 Center for Biological Sequence Analysis
30 Fungal Biodiversity Centre
31 Fungal Genetics and Technology Consultancy
2 TU Delft - Delft University of Technology
3 School of Biology
4 DTU - Danmarks Tekniske Universitet = Technical University of Denmark
5 WUR - Wageningen University and Research Centre
6 Department of Molecular Biology and Biotechnology
7 Universiteit Utrecht / Utrecht University [Utrecht]
8 Biomax Informatics AG
9 CLC bio
10 School of Biological Sciences
11 UGENT - Universiteit Gent = Ghent University
12 Flanders Institute for Biotechnology
13 University of Manchester [Manchester]
14 AFMB - Architecture et fonction des Macromolécules Biologiques - UMR 6098
15 Laboratory of Genetics
16 University of Groningen [Groningen]
17 BPF - Biologie et Pathogénicité fongiques
18 IP - Institut Pasteur [Paris]
19 Centre for Carbohydrate Bioprocessing
20 VU - Vrije Universiteit Amsterdam [Amsterdam]
21 Unité de recherche Pathologie végétale et phytobactériologie
22 Universiteit Leiden = Leiden University
23 Radboud University Medical Center [Nijmegen]
24 Vienna University of Technology
25 Qiagen GmbH
26 CMB - Center for Microbial Biotechnology
27 University of Szeged [Szeged]
28 HZI - Helmholtz Centre for Infection Research
29 Center for Biological Sequence Analysis
30 Fungal Biodiversity Centre
31 Fungal Genetics and Technology Consultancy
Etienne Danchin
- Fonction : Auteur
- PersonId : 734755
- IdHAL : danchin-etienne-gj
- ORCID : 0000-0003-4146-5608
- IdRef : 033644780
Christophe D'Enfert
- Fonction : Auteur
- PersonId : 801320
- ORCID : 0000-0002-6235-3886
- IdRef : 073388890
Thomas Guillemette
- Fonction : Auteur
- PersonId : 1151087
- IdHAL : thomas-guillemette
- ORCID : 0000-0002-5649-974X
Bernard Henrissat
- Fonction : Auteur
- PersonId : 833340
Xin Lu
- Fonction : Auteur
- PersonId : 775398
- ORCID : 0000-0002-6228-1480
- IdRef : 258563117
Jens Nielsen
- Fonction : Auteur
- PersonId : 758788
- ORCID : 0000-0002-9955-6003
Résumé
The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid. We sequenced the 33.9-megabase genome of A. niger CBS 513.88, the ancestor of currently used enzyme production strains. A high level of synteny was observed with other aspergilli sequenced. Strong function predictions were made for 6,506 of the 14,165 open reading frames identified. A detailed description of the components of the protein secretion pathway was made and striking differences in the hydrolytic enzyme spectra of aspergilli were observed. A reconstructed metabolic network comprising 1,069 unique reactions illustrates the versatile metabolism of A. niger. Noteworthy is the large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors, and the presence of putative gene clusters for fumonisin and ochratoxin A synthesis.