Lactobacilli are very ubiquitous, frequently associated to h uman microbial flora and foodstuffs ; where they have being observed to accomplish various protective roles against adverse microorganisms. A human strain of Lactobacillus paracasei subsp. paracasei originally isolated from newborn faeces was investigated o n its anti - listerial activities. Using intestinal Caco - 2 cell line in an in vitro model and abiotic surfaces, stainless steel and Teflon (polytetrafluoroethylene) which are the most largely used materials in food ind ustry, i t was observed that this strain exhibited adherence and anti - adherence properties. The inhibitory effects of this strain on the adherence of Listeria monocytogenes were determined. A decrease in the number of adhering pathogen cells was observed, using either pre - incubation or co - incubat ion of the pathogen with Lb. paracasei . Moreover, the anti - listerial and anti - adherence activities of its cell - free supernatant were examined. A n antibacterial activity related to the production of a bacteriocin - like substance and a d isplacement of the pat hogen from all the surfaces (Caco - 2 cell line, stainless steel and Teflon ) were registered. Together, these findings suggest that this strain could be used to prevent colonization of the gastrointestinal tract and the food - contact surfaces by L isteria mono cytogenes.