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Article Dans Une Revue Protein Engineering, Design and Selection Année : 2014

A web-based tool for rational screening of mutants libraries using ProSAR

Résumé

In directed evolution experiments, it is at stake to have methods to screen efficiently the mutant libraries. We propose a web-based tool that implements an established in silico method for the rational screening of mutant libraries. The method, known as ProSAR, attempts to link sequence data to activity. The method uses statistical models trained on small experimental datasets provided by the user. These can integrate potential epistatic interactions between mutations and be used in many diverse biological contexts. It drastically improves the search for leading mutants. The tool is freely available to non-commercial users at http://bo-protscience.fr/prosar/.
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Dates et versions

hal-01471908 , version 1 (22-05-2019)

Identifiants

Citer

Magali Berland, Bernard Offmann, Isabelle André, Magali Remaud Simeon, Philippe Charton. A web-based tool for rational screening of mutants libraries using ProSAR. Protein Engineering, Design and Selection, 2014, 27 (10), pp.375 - 381. ⟨10.1093/protein/gzu035⟩. ⟨hal-01471908⟩
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