A novel platform based on immobilized histidine tagged olfactory receptors, for the amperometric detection of an odorant molecule characteristic of boar taint - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Food Chemistry Année : 2015

A novel platform based on immobilized histidine tagged olfactory receptors, for the amperometric detection of an odorant molecule characteristic of boar taint

Résumé

We report a dose-dependent detection of androstenone in solution, as one of the boar taint compounds, based on related OR7D4 olfactory receptors immobilized on a gold electrode through their 6-His tag and NTA-copper complex, as visualized through fluorescence microscopy. Square wave voltammetry (SWV) is for the first time, the method used to monitor the olfactory receptor/odorant recognition process. The relative variation of the Cu(I)-Cu(II) current peak increases linearly versus log (concentration of androstenone) from 10(-14) M to 10(-4) M, in buffer solution. Negative tests were performed, using an unrelated odorant, helional, itself a ligand of OR 1740. Cross-selectivity was also tested after immobilization of OR 1740.
Fichier non déposé

Dates et versions

hal-01186656 , version 1 (25-08-2015)

Identifiants

Citer

Zhenzhong Guo, Nadia Zine, Florence Lagarde, Julien Daligault, Marie-Annick Persuy, et al.. A novel platform based on immobilized histidine tagged olfactory receptors, for the amperometric detection of an odorant molecule characteristic of boar taint. Food Chemistry, 2015, 184, pp.1-6. ⟨10.1016/j.foodchem.2015.03.066⟩. ⟨hal-01186656⟩
121 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More