Transcriptome analysis of chicken ES, blastodermal and germ cells reveals that chick ES cells are equivalent to mouse ES cells rather than EpiSC - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Stem Cell Research Année : 2015

Transcriptome analysis of chicken ES, blastodermal and germ cells reveals that chick ES cells are equivalent to mouse ES cells rather than EpiSC

Christian Jean
  • Fonction : Auteur
Nidia M.M. Oliveira
  • Fonction : Auteur
Sittipon Intarapat
  • Fonction : Auteur
Aurélie Fuet
  • Fonction : Auteur
  • PersonId : 982746
Irene de Almeida
  • Fonction : Auteur
Katherine Trevers
  • Fonction : Auteur
Sharon Boast
  • Fonction : Auteur
Pauline Aubel
  • Fonction : Auteur
Bertrand Pain

Résumé

Pluripotent Embryonic Stem cell (ESC) lines can be derived from a variety of sources. Mouse lines derived from the early blastocyst and from primordial germ cells (PGCs) can contribute to all somatic lineages and to the germ line, whereas cells from slightly later embryos (EpiSC) no longer contribute to the germ line. In chick, pluripotent ESCs can be obtained from PGCs and from early blastoderms. Established PGC lines and freshly isolated blastodermal cells (cBC) can contribute to both germinal and somatic lineages but established lines from the former (cESC) can only produce somatic cell types. For this reason, cESCs are often considered to be equivalent to mouse EpiSC. To define these cell types more rigorously, we have performed comparative microarray analysis to describe a transcriptomic profile specific for each cell type. This is validated by real time RT-PCR and in situ hybridisation. We find that both cES and cBC cells express classic pluripotency-related genes (including cPOUV/OCT4, NANOG, SOX2/3, KLF2 and SALL4), whereas expression of DAZL, DND1, DDX4 and PIWIL1 defines a molecular signature for germ cells. Surprisingly, contrary to the prevailing view, our results also suggest that cES cells resemble mouse ES cells more closely than mouse EpiSC.
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hal-02372014 , version 1 (27-05-2020)

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Christian Jean, Nidia M.M. Oliveira, Sittipon Intarapat, Aurélie Fuet, Clément Mazoyer, et al.. Transcriptome analysis of chicken ES, blastodermal and germ cells reveals that chick ES cells are equivalent to mouse ES cells rather than EpiSC. Stem Cell Research, 2015, 14 (1), pp.54-67. ⟨10.1016/j.scr.2014.11.005⟩. ⟨hal-02372014⟩
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