Measurement of cell traction forces with ImageJ. - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Chapitre D'ouvrage Année : 2015

Measurement of cell traction forces with ImageJ.

Résumé

The quantification of cell traction forces requires three key steps: cell plating on a deformable substrate, measurement of substrate deformation, and the numerical estimation of the corresponding cell traction forces. The computing steps to measure gel deformation and estimate the force field have somehow limited the adoption of this method in cell biology labs. Here we propose a set of ImageJ plug-ins so that every lab equipped with a fluorescent microscope can measure cell traction forces.
Fichier non déposé

Dates et versions

hal-01142591 , version 1 (15-04-2015)

Identifiants

Citer

Jean-Louis Martiel, Aldo Leal, Laetitia Kurzawa, Martial Balland, Irene Wang, et al.. Measurement of cell traction forces with ImageJ.. Paluch, E.K. Methods in Cell Biology, 125, Elsevier, pp.269-87, 2015, Biophysical methods in cell biology, 978-0-12-801103-4. ⟨10.1016/bs.mcb.2014.10.008⟩. ⟨hal-01142591⟩
379 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More