An investigation of in situ N mineralization, using undisturbed soil samples, indicated a negative relationship between the mineral N content [(NO3+NH4)-N] at the beginning of the experiment and the mineral N produced during it. This suggests that a maximum value of mineral N accumulation in intact soil cores could be calculated from the relationship between mineral N content and N mineralization rate. This value would be related to the size of the mineralizable N pool. If this hypothesis is true, the amount of mineralizable N could be estimated from in situ incubations and utilized in the modelling of N mineralization in the field. The aim of this work was to verify this hypothesis. The relationship between the mineral N content and the N mineralization rate was analysed for in situ and laboratory incubations of disturbed and undisturbed soil samples. A negative relationship between the two variables was only obtained for the experiments carried out with undisturbed samples (in the field and laboratory incubations) when the soil moisture content was not limiting for N mineralization. Futhermore, in undisturbed samples, a negative relationship between mineralization rates of consecutive incubation periods was observed, i.e. the soil sample producing relatively more, during a given period, produced relatively less in the following period. This relationship suggests a feedback mechanism operating in N mineralization which would be related to a mineralization-immobilization process in soil microsites. Thus, the N mineralization pattern was more complex than that described by initial hypothesis. The possible consequence of this feedback mechanism on in situ N dynamics is discussed.