The chemical and biological survey has been achieved on the stems of Erythrophleum africanum (Fabaceae), Terminalia albida (Combretaceae) and Zanthoxylum zanthoxyloides (Rutaceae), from 3 African plants used as toothpicks. The mineral quantification using the scanning electron microscope, highlight the presence of Al, Cl, Fe, K, Mg, Mn, P, S, Si with a relative abundance in K and Ca, when the organic characterization by UPLC revealed the existence of phytophenols notably epigallocatechin gallate (EGCG), epicatechin gallate (ECG), chlorogenic acid, gallic acid, burkinabines, ellagitannins). Compared to vitamin C and butylhydroxytoluene (BHT), hydroacetonic extract from E. africanum stem exhibited a significant antiradical power (92.19 %) with regard to DPPH. The biological screening of the aforesaid extract showed an antibacterial activity against Staphylococcus aureus and Micrococcus luteus. The hydroacetonic extracts from E. africanum, T. albida and Z. zanthoxyloides stems signed an activity with regard to 6 cancerous strains MDA-MB 231, Caco-2s, B16F10, MDA-MB 435, SNB75 and C6, with a good antiproliferative profile opposite Caco-2, SNB75 and of C6. Among the extracts of the drugs tested, the one of Z. zanthoxyloides stem made notice a more important effect on all cancerous strains.