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Article Dans Une Revue Journal of Microbiological Methods Année : 2018

A standardized toolkit for genetic engineering of CTG clade yeasts

Marc Clastre
Arnaud Lanoue
Christophe d'Enfert

Résumé

We have developed a series of synthetic constructs suitable to genetically manipulate a broad range of yeast species belonging to the fungal CTG clade. This molecular toolbox notably allows heterologous gene expression, single or dual fluorescence labeling and construction of luciferase-expressing strains for bioluminescence imaging.
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Dates et versions

hal-02406599 , version 1 (12-12-2019)

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Citer

Tatiana Defosse, Vincent Courdavault, Alix Coste, Marc Clastre, Thomas Duge de Bernonville, et al.. A standardized toolkit for genetic engineering of CTG clade yeasts. Journal of Microbiological Methods, 2018, 144, pp.152-156. ⟨10.1016/j.mimet.2017.11.015⟩. ⟨hal-02406599⟩
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