Sox5 is involved in germ-cell regulation and sex determination in medaka following co-option of nested transposable elements - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue BMC Biology Année : 2018

Sox5 is involved in germ-cell regulation and sex determination in medaka following co-option of nested transposable elements

Résumé

Background - Sex determination relies on a hierarchically structured network of genes, and is one of the most plastic processes in evolution. The evolution of sex-determining genes within a network, by neo- or sub-functionalization, also requires the regulatory landscape to be rewired to accommodate these novel gene functions. We previously showed that in medaka fish, the regulatory landscape of the master male-determining gene dmrt1bY underwent a profound rearrangement, concomitantly with acquiring a dominant position within the sex-determining network. This rewiring was brought about by the exaptation of a transposable element (TE) called Izanagi, which is co-opted to act as a silencer to turn off the dmrt1bY gene after it performed its function in sex determination. Results - We now show that a second TE, Rex1, has been incorporated into Izanagi. The insertion of Rex1 brought in a preformed regulatory element for the transcription factor Sox5, which here functions in establishing the temporal and cell-type-specific expression pattern of dmrt1bY. Mutant analysis demonstrates the importance of Sox5 in the gonadal development of medaka, and possibly in mice, in a dmrt1bY-independent manner. Moreover, Sox5 medaka mutants have complete female-to-male sex reversal. Conclusions - Our work reveals an unexpected complexity in TE-mediated transcriptional rewiring, with the exaptation of a second TE into a network already rewired by a TE. We also show a dual role for Sox5 during sex determination: first, as an evolutionarily conserved regulator of germ-cell number in medaka, and second, by de novo regulation of dmrt1 transcriptional activity during primary sex determination due to exaptation of the Rex1 transposable element.
Fichier principal
Vignette du fichier
2018_Schartl_BMC Biology_1.pdf (3.07 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Loading...

Dates et versions

hal-02621207 , version 1 (26-05-2020)

Licence

Paternité

Identifiants

Citer

Manfred Schartl, Susanne Schories, Yuko Wakamatsu, Yusuke Nagao, Hisashi Hashimoto, et al.. Sox5 is involved in germ-cell regulation and sex determination in medaka following co-option of nested transposable elements. BMC Biology, 2018, 16:16 (1), pp.1-17. ⟨10.1186/s12915-018-0485-8⟩. ⟨hal-02621207⟩
58 Consultations
38 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More