The nanos1 gene was duplicated in early Vertebrates and the two paralogs show different gonadal expression profiles in a shark - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2018

The nanos1 gene was duplicated in early Vertebrates and the two paralogs show different gonadal expression profiles in a shark

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Nanos are RNA-binding proteins playing crucial roles in germ cell development and maintenance.Based on phylogenetic and synteny analyses, this study reveals that nanos1 gene has undergonemultiple duplications and gene copies losses in Vertebrates. Chondrichthyan species display twonanos1 genes (named nanos1A/1B), which were both retrieved in some Osteichthyes at basal positionsin Sarcopterygii and Actinopterygii lineages. In contrast, Teleosts have lost nanos1A but duplicatednanos1B leading to the emergence of two ohnologs (nanos1Ba/1Bb), whereas Tetrapods have lostnanos1B gene. The two successive nanos gene duplications may result from the second and third wholegenome duplication events at the basis of Vertebrates and Teleosts respectively. The expression proflesof nanos1A and nanos1B paralogs were characterized in the dogfsh, Scyliorhinus canicula. Nanos1A wasstrongly expressed in brain and also localized in all germ cell types in the polarized testis. In contrast,nanos1B was detected in testis with the highest expression in the germinative zone. In addition,Nanos1B protein was predominantly located in the nuclei of male germinal cells. In the ovary, bothparalogs were detected in germinal and somatic cells. Our study opens new perspectives concerning thecomplex evolution of nanos1 paralogs and their potential distinct roles in Vertebrates gonads.
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hal-01791808 , version 1 (14-05-2018)

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Laura Gribouval, Pascal Sourdaine, Jean-Jacques Lareyre, Johanna Bellaïche, Florence Le Gac, et al.. The nanos1 gene was duplicated in early Vertebrates and the two paralogs show different gonadal expression profiles in a shark. Scientific Reports, 2018, 8:6942 (1), pp.1-14. ⟨10.1038/s41598-018-24643-1⟩. ⟨hal-01791808⟩
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