Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Chapitre D'ouvrage Année : 2018

Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains

Résumé

In this chapter, we describe different methods for phenotyping strains or mutants of the bacterial wilt agent, Ralstonia solanacearum, on four different host plants: Arabidopsis thaliana, tomato (Solanum lycopersicum), tobacco (Nicotiana benthamiana), or Medicago truncatula. Methods for preparation of high volume or low volume inocula are first described. Then, we describe the procedures for inoculation of plants by soil drenching, stem injection or leaf infiltration, and scoring of the wilting symptoms development. Two methods for measurement of bacterial multiplication in planta are also proposed: (1) counting the bacterial colonies upon serial dilution plating and (2) determining the bacterial concentration using a qPCR approach. In this chapter, we also describe a competitive index assay to compare the fitness of two strains coinoculated in the same plant. Lastly, specific protocols describe in vitro and hydroponic inoculation procedures to follow disease development and bacterial multiplication in both the roots and aerial parts of the plant.
Fichier principal
Vignette du fichier
MMiB Morel 2018.pdf (567.5 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-02791793 , version 1 (22-07-2022)

Licence

Copyright (Tous droits réservés)

Identifiants

Citer

Arry Morel, Nemo Peeters, Fabienne Vailleau, Patrick Barberis, Gaofey Jiang, et al.. Plant Pathogenicity Phenotyping of Ralstonia solanacearum Strains. Host-Pathogen Interactions: Methods and Protocols, 1734, Carlos Medina,Francisco Javier López-Baena, 2018, Methods in Molecular Biology, 978-1-4939-7604-1; 978-1-4939-7603-4. ⟨10.1007/978-1-4939-7604-1_18⟩. ⟨hal-02791793⟩
29 Consultations
104 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More