Next-Generation Sequencing Accelerates Crop Gene Discovery - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Trends in Plant Science Année : 2019

Next-Generation Sequencing Accelerates Crop Gene Discovery

Résumé

The identification and isolation of genes underlying quantitative trait loci (QTLs) associated with agronomic traits in crops have been recently accelerated thanks to next-generation sequencing (NGS)-based technologies combined with plant genetics. With NGS, various revisited genetic approaches, which benefited from higher marker density, have been elaborated. These approaches improved resolution in QTL position and assisted in determining functional causative variations in genes. Examples of QTLs/genes associated with agronomic traits in crops and identified using different strategies based on whole-genome sequencing (WGS)/whole-genome resequencing (WGR) or RNA-seq are presented and discussed in this review. More specifically, we summarize and illustrate how NGS boosted bulk-segregant analysis (BSA), expression profiling, and the construction of polymorphism databases to facilitate the detection of QTLs and causative genes.
Fichier principal
Vignette du fichier
S136013851830270X.pdf (809.13 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-02625789 , version 1 (22-10-2021)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

- Khanh Le Nguyen, Alexandre Grondin, Brigitte Courtois, Pascal Gantet. Next-Generation Sequencing Accelerates Crop Gene Discovery. Trends in Plant Science, 2019, 24 (3), pp.263-274. ⟨10.1016/j.tplants.2018.11.008⟩. ⟨hal-02625789⟩
32 Consultations
435 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More