Arterio-venous metabolomics exploration reveals major changes across liver and intestine in the obese Yucatan minipig - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Scientific Reports Année : 2019

Arterio-venous metabolomics exploration reveals major changes across liver and intestine in the obese Yucatan minipig

Nathalie Poupin
Cécile Canlet
Didier Remond
Laurent Debrauwer
Dominique Dardevet
Fabien Jourdan

Résumé

Blood circulation mainly aims at distributing the nutrients required for tissue metabolism and collecting safely the by-products of all tissues to be further metabolized or eliminated. The simultaneous study of arterial (A) and venous (V) specific metabolites therefore has appeared to be a more relevant approach to understand and study the metabolism of a given organ. We propose to implement this approach by applying a metabolomics (NMR) strategy on paired AV blood across the intestine and liver on high fat/high sugar (HFHS)-fed minipigs. Our objective was to unravel kinetically and sequentially the metabolic adaptations to early obesity/insulin resistance onset specifically on these two tissues. After two months of HFHS feeding our study of AV ratios of the metabolome highlighted three major features. First, the hepatic metabolism switched from carbohydrate to lipid utilization. Second, the energy demand of the intestine increased, resulting in an enhanced uptake of glutamine, glutamate, and the recruitment of novel energy substrates (choline and creatine). Third, the uptake of methionine and threonine was considered to be driven by an increased intestine turnover to cope with the new high-density diet. Finally, the unique combination of experimental data and modelling predictions suggested that HFHS feeding was associated with changes in tryptophan metabolism and fatty acid β-oxidation, which may play an important role in lipid hepatic accumulation and insulin sensitivity.
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hal-02282295 , version 1 (09-09-2019)

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Nathalie Poupin, Marie Tremblay-Franco, Aurélien Amiel, Cécile Canlet, Didier Remond, et al.. Arterio-venous metabolomics exploration reveals major changes across liver and intestine in the obese Yucatan minipig. Scientific Reports, 2019, 9 (1), ⟨10.1038/s41598-019-48997-2⟩. ⟨hal-02282295⟩
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