Intravaginal insemination depth influences fertility outcomes in Indian red jungle fowl (Gallus gallus murghi) - INRAE - Institut national de recherche pour l’agriculture, l’alimentation et l’environnement Accéder directement au contenu
Article Dans Une Revue Animal Biotechnology Année : 2021

Intravaginal insemination depth influences fertility outcomes in Indian red jungle fowl (Gallus gallus murghi)

Résumé

The depth of intravaginal insemination to achieve optimum fertility with frozen-thawed semen is highly species specific in birds and differ even in breed and/or strains of a species. Therefore, study was designed to evaluate the influence of intravaginal insemination depths (2 and 4 cm) on fertility outcome in Indian red jungle fowl. Semen collected from eight mature cocks was pooled, diluted in extender and cooled to 4 degrees C. Glycerol (20%) was added to chilled semen, equilibrated for 10 min and cryopreserved. After 3 days of storage, frozen semen was thawed in water bath at 37 degrees C for 30 s. After glycerol removal, intravaginal Inseminations were performed at the depth of 2 and 4 cm. The no. of fertilized eggs (31.4 +/- 1.6 vs. 27.7 +/- 1.8), fertility rate (65.7 +/- 3.6 vs. 58.8 +/- 4.0), no. of hatched chicks (27.8 +/- 1.9 vs. 23.5 +/- 1.6), hatchability of set eggs (58.8 +/- 4.3 vs. 49.7 +/- 3.2) and hatchability of fertilized eggs (88.4 +/- 2.8 vs. 84.3 +/- 2.2) were recorded higher with intravaginal depth of 4 cm compared to 2 cm. It is concluded that intravaginal insemination at the depth of 4 cm enhances the fertility outcomes of the frozen-thawed Indian red jungle fowl semen.
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Dates et versions

hal-02622711 , version 1 (26-05-2020)

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Citer

Bushra A. Rakha, Muhammad S. Ansari, Shamim Akhter, Ali Akhter, Elisabeth Blesbois. Intravaginal insemination depth influences fertility outcomes in Indian red jungle fowl (Gallus gallus murghi). Animal Biotechnology, 2021, 32 (4), pp.526-530. ⟨10.1080/10495398.2020.1714634⟩. ⟨hal-02622711⟩
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