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Article Dans Une Revue Metabolic Engineering Année : 2011

Evolutionary engineered Saccharomyces cerevisiae wine yeast strains with increased in vivo flux through the pentose phosphate pathway

Axelle Cadiere
Carole Camarasa
Sylvie S. Dequin

Résumé

Amplification of the flux toward the pentose phosphate (PP) pathway might be of interest for various S. cerevisiae based industrial applications. We report an evolutionary engineering strategy based on a long-term batch culture on gluconate, a substrate that is poorly assimilated by S. cerevisiae cells and is metabolized by the PP pathway. After adaptation for various periods of time, we selected strains that had evolved a greater consumption capacity for gluconate. (13)C metabolic flux analysis on glucose revealed a redirection of carbon flux from glycolysis towards the PP pathway and a greater synthesis of lipids. The relative flux into the PP pathway was 17% for the evolved strain (ECA5) versus 11% for the parental strain (EC1118). During wine fermentation, the evolved strains displayed major metabolic changes, such as lower levels of acetate production, higher fermentation rates and enhanced production of aroma compounds. These represent a combination of novel traits, which are of great interest in the context of modern winemaking.

Dates et versions

hal-02650614 , version 1 (29-05-2020)

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Citer

Axelle Cadiere, Anne Ortiz-Julien, Carole Camarasa, Sylvie S. Dequin. Evolutionary engineered Saccharomyces cerevisiae wine yeast strains with increased in vivo flux through the pentose phosphate pathway. Metabolic Engineering, 2011, 13 (3), pp.263-271. ⟨10.1016/j.ymben.2011.01.008⟩. ⟨hal-02650614⟩
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