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Communication Dans Un Congrès Année : 2010

Red blood cell levels of choline-phospholipids as a mirror of retinal and optic nerve compositions: Human data

Résumé

Purpose:The lipid composition of red blood cell membranes is currently admitted to reflect that of nervous tissues, including the retina and the optic. nerve. We have shown previously specific alterations of red blood cell levels of individual species of phosphatidyl-cholines (PC) and plasmenyl-cholines. (PlsC) in primary open angle glaucoma (POAG) patients (Acar et al, Exp Eye Res 2009: 840-853). In order to assign these circulating modifications to. ocular changes, the aim of this work was to identify in humans, plausible associations in the levels PC and PlsC between red blood cells, retina and optic. nerve. Methods:Red blood cells, retinas and optic nerves were collected from nine donors (body donation to Science). Following lipid extraction, total. phospholipids were isolated using silica cartridges. The individual species of PC and PlsC were quantified by HPLC-ESI-MS/MS on a triple quadrupole. MS instrument. Correlations were estimated for each compound to determine plausible associations between circulating and tissue concentrations. Results:Several associations were found between red blood cell and retinal and/or optic nerve levels of individual species of PC and PlsC. For PlsC, the. associations were mainly with the optic nerve whereas they were restricted to the retina for PC. PlsC18:1/22:4 in red blood cells was positively. associated with PlsC18:1/16:0 in the optic nerve (r=0.75, p=0.04) and PlsC18:0/22:4 with PlsC18:1/22:4 and PlsC18:1/20:2 (r=0.82, p=0.02 and r=0.91,. p=0.004, respectively). On the other hand, PC18:0/22:6 in red blood cells was positively associated with PC18:1/22:6 and PC22:6/22:6 in the retina. (r=0.67, p=0.003 and r=0.74, p=0.01, respectively). Conclusions:The red blood cell concentrations of some individual species of PlsC and PC appear to reflect their levels in the optic nerve and in the. retina, respectively. Since PlsC are known to be concentrated in myelin and PC in retinal cells, the alteration of their levels in red blood cells of POAG. patients may suggest optic nerve demyelinisation and retinal cell loss processes.

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Dates et versions

hal-00904315 , version 1 (03-06-2020)

Identifiants

  • HAL Id : hal-00904315 , version 1
  • PRODINRA : 50714

Citer

Niyazi Acar, Olivier Berdeaux, Lucy Martine, Gilles Thuret, Stéphanie Cabaret, et al.. Red blood cell levels of choline-phospholipids as a mirror of retinal and optic nerve compositions: Human data. Association for Research in Vision and Ophthalmology annual congress, Labo/service de l'auteur, Ville service, Pays service., May 2009, Fort Lauderdale (Florida), United States. pp.E-Abstract 6124. ⟨hal-00904315⟩
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