An increasing number of childbearing age women are overweight or obese. Current recommendations include a preconceptional weight loss (WL) of 5 to 10% to reach a normal pre-pregnancy body mass index. Although there is evidence that preconceptional WL reduces pregnancy complications but can induce growth retardation or preterm delivery , the long-term consequences on offspring health remain unknown. ln the light of the Developmental Origins of Health and Disease (DOHaD) hypothesis, maternal pre-pregnancy weight trajectories could have beneficial, neutral or detrimental long-term effects on offspring• Our aim was to investigate the outcomes of preconception WL in obese C57BL/6J female mice on offspring susceptibility to an obesogenic diet in later life. Female mice of the WL group were led a High-Fat Diet (HFD) for two months and a control diet (CD) for the following two months. Two control groups consisted in: Lean and Obese Control (LC and OC), receiving either the CD or HFD diet for four months and during gestation/lactation. At weaning, offspring were led either a HFD or a CD. Weight, calorie intake, glucose metabolism and body composition were followed until sacrifice at 6 months. No significant differences were observed preweaning between LC and WL offspring for weight and glycaemia. As for OC offspring, they had a higher body weight and lasting hyperglycemia. Sexual dimorphism was observed alter weaning for all parameters studied and as adults, all HFD-fed offspring had an increased body weight, adiposity and lasting hyperglycemia, independently of maternal group. ln our model, the offspring phenotype relies mainly on the postweaning diet. Despite abundant literature on in utero exposure to a one paper reported the absence of association between offspring parameters and preconception HFD. More investigations are needed to complete this phenotyping and at epigenetic levels to draw conclusions about maternal preconception trajectory effects on offspring health.