The ecological consequences of seed size variation have been studied extensively in plants. Curiously, little attention has been paid to the qualitative and quantitative variation of the seed-stored molecules and on their ecological significance. Here, we analysed the oil content and oil composition of ca. 200 weed seed species from agricultural fields in France based on single seed accessions, concentrating on interspecies differences and ignoring within-species variation. The relationships between seed weight, oil %, fatty acids (FAs) and the energetic value of the seed and its antioxidant properties were also investigated. The antioxidant activity could contribute to protect the oily seed reserves from alteration over time. Among the species analysed, we found a considerable quantitative (oil%) and qualitative variation of FAs stored in the seeds. Such variation was largely related to the plant family of the different species, but intrafamily variation was also found. Heavier seeds contained less oil on a per gram basis than lighter seeds, suggesting a trade-off between seed weight and oil ratio in the seed and that oil storage strategy depends on seed size. Moreover, oily seeds contained more polyunsaturated FAs. However, contrary to our hypothesis, we did not found a higher antioxidant capability in oily seed extracts than in non-oily seeds, nor to the quantitative or to the qualitative variation of FAs in the seeds. Considering the role of these important trait variations on weed ecological strategies, such as germination period, seed predation rate and competition–colonisation trade-off, could improve the sustainable management of weed communities.