PURIFICATION OF GONADOTROPIN FROM RAINBOW TROUT (SALMO GAIRDNERII RICHARDSON) PITUITARY GLANDS
Résumé
A method of purifying trout gonadotropic hormone is discussed. The technique consists of the following stages : alcoholic extraction, two successive gel filtrations on Sephadex G5o- and G-ioo, chromatography on DEAE-cellulose and ultrogel A¢SC.A The biological activity of fractions obtained is measured using as assay the maturation in vitro of trout oocytes. The preparation obtained has a molecular weight of about 2,7500 determined by behavior on Sephadex-G 1 00 and analytical ultracentrifugation for KS20Sw of 2.45 Polyacrylamide gel electrophoresis shows two strips of electrophoretic mobility, of 0.50 and 0.58. Analysis of the amino acid composition of the preparation indicates a number of 160 AA residues per mole. These different values are compared to the corresponding ones for c-GtH, s-GtH and mammalian gonadotropins.
Origine : Accord explicite pour ce dépôt
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