Characterizing a region on BTA11 affecting β-lactoglobulin content of milk using high-density genotyping and haplotype grouping
Résumé
Background: Milk β-lactoglobulin (β-LG) content is of interest as it is associated with nutritional and manufacturing
properties. It is known that milk β-LG content is strongly affected by genetic factors. In cattle, most of the genetic
differences are associated with a chromosomal region on BTA11, which contains the β-LG gene. The aim of this
study was to characterize this region using 777 k SNP data (BovineHDbeadChip) and perform a haplotype-based
association study. A statistical approach was developed to build haplotypes that capture the genetic variation
associated with this genomic region.
Results: The SNP with the most significant effect on β-lactoglobulin content was one of the 2 causal mutations
responsible for the β-lactoglobulin protein variants A/B. Haplotypes based on 2 to 5 selected lead SNP were
clustered in groups with different effects on β-lactoglobulin content. Four different groups were identified
suggesting that β-lactoglobulin variant A and B can be further refined in A1, A2, B1 and B2.
Conclusions: This study showed that β-lactoglobulin protein variants A/B do not explain all genetic variation
associated with the tail part of BTA11 but this region contains more than one mutation with an effect on
β-lactoglobulin content. These findings can be used for selection of cows with higher cheese yield, which is
desirable for the dairy industry.
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